【摘要】：目的對三種不同的檢測BALB/c小鼠是否受孕的方法進(jìn)行比較研究。方法只檢測陰栓(方法一)、檢測陰栓結(jié)合稱量體質(zhì)量(方法二)、只稱量體質(zhì)量(方法三),用3種方法檢測同一組雌鼠的受孕情況并比較各方法的檢測能力。結(jié)果方法一檢測小鼠受孕的靈敏度為0.71、特異度為0.47,方法二靈敏度為0.71、特異度為0.94,方法三靈敏度為0.96、特異度為0.94。結(jié)論在對受孕(gestation day,GD)10天及后期孕鼠的檢測方法中,稱量體質(zhì)量較其它方法更為可靠和實用。
【作者單位】： 山西醫(yī)科大學(xué)第一醫(yī)院口腔科;
【關(guān)鍵詞】： BALB/c小鼠 受孕 檢測方法
【基金】：山西省自然科學(xué)基金2013011056-5
【分類號】：S865.13
【正文快照】：
在應(yīng)用小鼠的實驗研究中,經(jīng)常需要在特定孕期以一定方式干預(yù)孕鼠,所以如何準(zhǔn)確檢測小鼠是否受孕成為進(jìn)行此類實驗常常面臨的問題。一般采用陰栓檢測法、陰道精子檢測法來確定小鼠是否受孕,但事實上該類方法只能確定小鼠進(jìn)行交配,并不能真實反映實際受孕情況[1]。另有學(xué)者采用
Abstract： Abstract:Objective To compare and study three inspecting methods which test whether the mice were pregnant or not． Method Check and study the fertilization of the same mice group with three methods． The first method was to use vaginal suppository to identify the mice were pregnant or not; the second method was to use vaginal suppository and check mice’s weight; the third method was to check mice’s weight． Ｒesult The first method sees the sensitivity of the mice’s pregnancy was 0. 71 and the specificity was 0. 47． According to the second method，the sensitivity was 0. 71 and the specificity was 0. 96． And third method shows that the sensitivity was 0. 96 and the specificity was 0. 94． Conclusion For the methods detecting mouse gestation GD 10 and late state，observing change of weight is more reliable and practical than other methods in the process of inspecting pregnant mouse in the GD 10 period and late stage．
Keywords： BALB / c mice pregnancy inspecting method
【作者單位】： 山西醫(yī)科大學(xué)第一醫(yī)院口腔科;
【關(guān)鍵詞】： BALB/c小鼠 受孕 檢測方法
【基金】：山西省自然科學(xué)基金2013011056-5
【分類號】：S865.13
【正文快照】：
在應(yīng)用小鼠的實驗研究中,經(jīng)常需要在特定孕期以一定方式干預(yù)孕鼠,所以如何準(zhǔn)確檢測小鼠是否受孕成為進(jìn)行此類實驗常常面臨的問題。一般采用陰栓檢測法、陰道精子檢測法來確定小鼠是否受孕,但事實上該類方法只能確定小鼠進(jìn)行交配,并不能真實反映實際受孕情況[1]。另有學(xué)者采用
Abstract： Abstract:Objective To compare and study three inspecting methods which test whether the mice were pregnant or not． Method Check and study the fertilization of the same mice group with three methods． The first method was to use vaginal suppository to identify the mice were pregnant or not; the second method was to use vaginal suppository and check mice’s weight; the third method was to check mice’s weight． Ｒesult The first method sees the sensitivity of the mice’s pregnancy was 0. 71 and the specificity was 0. 47． According to the second method，the sensitivity was 0. 71 and the specificity was 0. 96． And third method shows that the sensitivity was 0. 96 and the specificity was 0. 94． Conclusion For the methods detecting mouse gestation GD 10 and late state，observing change of weight is more reliable and practical than other methods in the process of inspecting pregnant mouse in the GD 10 period and late stage．
Keywords： BALB / c mice pregnancy inspecting method
